Zlatkis, Albert2022-02-092022-02-0919784727762https://hdl.handle.net/10657/8710A concentration technique for the analysis of mono- and oligosaccharides by gas chromatography has been developed. The method increases the detection limit up to 500 times by using a double phase extraction of silylated derivatives and a splitless injection. The method has been found to be fast and reproducible for a variety of samples. The determination included liquid, solid and semisolid samples. Some samples of biological origin as cell cultures and yeasts were determined. The method was found particularly useful in the taxonomic classification of four strains of yeast. Headspace sampling was applied to the analysis of volatiles from several yeast strains and the results compared. Some differences in the profiles were found. The transevaporation technique was applied to the analysis of volatiles from cell cultures.application/pdfenThis item is protected by copyright but is made available here under a claim of fair use (17 U.S.C. §107) for non-profit research and educational purposes. Users of this work assume the responsibility for determining copyright status prior to reusing, publishing, or reproducing this item for purposes other than what is allowed by fair use or other copyright exemptions. Any reuse of this item in excess of fair use or other copyright exemptions requires express permission of the copyright holder.Thesisreformatted digital