Polymeric Signal Amplifiers in Biosensing

dc.contributor.advisorWu, Tianfu
dc.contributor.committeeMemberMohan, Chandra
dc.contributor.committeeMemberMajd, Sheereen
dc.contributor.committeeMemberShevkoplyas, Sergey S.
dc.contributor.committeeMemberBao, Jiming
dc.creatorLin, Zuan-Tao
dc.creator.orcid0000-0001-5054-3773
dc.date.accessioned2019-05-23T15:45:27Z
dc.date.createdAugust 2018
dc.date.issued2018-08
dc.date.submittedAugust 2018
dc.date.updated2019-05-23T15:45:27Z
dc.description.abstractEarly detection of ultra-low concentrations of biomarkers or environmental toxins in bodyfluids, food or water samples is vital for disease diagnosis, food safety and environmental monitoring. However, traditional technologies are tedious, time-consuming and require expensive instruments and well-trained personnel. Thus, there is an increasing demand to develop a facile and rapid sensing strategy to overcome these limitations. Signal amplification is important in early detection. Among these emerging signal amplification methods, polymer based signal amplification is promising due to the structure of polymer and easy chemical modification. In this topic, we developed four types of bioseners for ultrasensitive detection of biomarker and toxin, as well as monitoring of cell growth. (1) We fabricated a novel signal cascade strategy via an ultrasensitive polymeric sensing system (UPSS) composed of gold nanoparticle (gNP)-decorated polymers, which enables gNP aggregation in polymeric network and electrical conductance change upon specific aptamer-based biomolecular recognition. The biomolecular recognition induced polymeric network shrinkage responses as well as dose-dependent responses of the UPSS are validated using in situ real-time atomic-force microscopy, representing the first instance of real-time detection of biomolecular binding-induced polymer shrinkage in soft matter. (2) We constructed an ultrasensitive biosensor where a C-reactive protein (CRP) aptamers based molecular recognition core, and an in situ synthesized conductive polypyrrole nanofiber mesh based signal amplifier were integrated. Serum CRP levels were quantitatively analyzed through monitoring the conductance change caused by polymeric network shrinkage upon the aptamer-CRP binding. A commercial CRP ELISA kit was used to perform side-by-side measurement of serum CRP in melanoma patients. (3) We prepared a dual-function polymer scaffold containing thermosensitive polymers, silver flakes-decorated polymers and gNP-decorated polymers to monitor cancer cells growth. This biocompatible polymer scaffold allows cancer cells to grow on it and can detect cancer cells via silver flakes and gNP aggregation in polymeric network and electrical conductance change upon the cancer cells growth. (4) We fabricated a poly(3,4-ethylenedioxythiophene):poly(styrene sulfonate) (PEDOT:PSS) based, highly-responsive flexible composite nanofiber signal amplifier for ultrasensitive detection of thrombin and IL-17 A. The serum IL-17 A in melanoma patients were quantified by this sensor.
dc.description.departmentBiomedical Engineering, Department of
dc.format.digitalOriginborn digital
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/10657/4021
dc.language.isoeng
dc.rightsThe author of this work is the copyright owner. UH Libraries and the Texas Digital Library have their permission to store and provide access to this work. Further transmission, reproduction, or presentation of this work is prohibited except with permission of the author(s).
dc.subjectPolymers
dc.subjectBiosensors
dc.subjectGold nanoparticles
dc.subjectPolypyrrole
dc.subjectPEDO:TPSS
dc.subjectSignal amplifer
dc.subjectSignal amplification
dc.titlePolymeric Signal Amplifiers in Biosensing
dc.type.dcmiText
dc.type.genreThesis
local.embargo.lift2020-08-01
local.embargo.terms2020-08-01
thesis.degree.collegeCullen College of Engineering
thesis.degree.departmentBiomedical Engineering, Department of
thesis.degree.disciplineBiomedical Engineering
thesis.degree.grantorUniversity of Houston
thesis.degree.levelDoctoral
thesis.degree.nameDoctor of Philosophy

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