Development of Polymeric Networks for Drug Delivery and Tissue Engineering Applications

dc.contributor.advisorHarth, Eva M.
dc.contributor.committeeMemberMay, Jeremy A.
dc.contributor.committeeMemberDaugulis, Olafs
dc.contributor.committeeMemberBaldelli, Steven
dc.contributor.committeeMemberRobertson, Megan L.
dc.creatorLoftin, Breyinn
dc.date.accessioned2022-06-18T23:44:14Z
dc.date.createdAugust 2021
dc.date.issued2021-08
dc.date.submittedAugust 2021
dc.date.updated2022-06-18T23:44:15Z
dc.description.abstractThe synthetic versatility of polymeric networks provide the opportunity to use organic and polymer chemistry for the precise synthesis of macromolecular building blocks to form a host of materials that can be tuned for specific biomedical applications. This dissertation focuses on the development of polymeric networks ranging in size from bulk materials as hydrogels, to discrete micron structures and nanoparticles for applications in tissue engineering and drug delivery. The development of a novel hydrogel material based on a suitable poly(ethylene glycol) (PEG) alternative, polyglycidol, is described. Incorporation of THF as a comonomer in the ring opening polymerization of glycidol reducing the branching in the structure providing a semi-branched architecture. Upon functionalization with ketone and aminooxy groups, these derivatives are brought together to produce hydrogels with great promise for tissue engineering applications. Later, poly(dimethyl acrylamide-co-diacetone acrylamide) copolymers were cross-linked with a variety of synthesized aminooxy-PEG cross-linkers to produce an oxime-based host hydrogel tuned in its hydrophilicity and elasticity. An interpenetrated network consisting of self-assembled peptides within the host hydrogel was established. Results demonstrated the self-assembled peptide network was exclusively responsible for triggering cell adhesion while the dense oxime-based network provided the mechanically stable environment required by the bioactive system. To further evolve nanoparticle platforms previously developed, a novel second-generation particle system was established to create particles ranging in size from the nanoscale to the microscale in a simple, robust one pot synthetic method. By utilizing acyclic diene metathesis (ADMET) polymerization, high molecular weight polyesters possessing backbone functionalities were synthesized for intermolecular cross-linking via thiol-Michael addition chemistry with a dithiol linker for particle formation. Variations in the degree of functionalization and reaction concentration led to particles tuned in their cross-linking density in well-defined size dimensions. This practical synthetic approach combined with tunable drug release profiles based on cross-linking density makes this system a promising platform for drug delivery applications. Lastly, to broaden the applications of this new particle platform for potential use in tissue engineering, progress towards incorporating these particles in organo/hydrogels as reinforcement agents to improve the overall mechanical properties of the network is discussed.
dc.description.departmentChemistry, Department of
dc.format.digitalOriginborn digital
dc.format.mimetypeapplication/pdf
dc.identifier.citationPortions of this document appear in: Criado-Gonzalez, Miryam, Breyinn Loftin, Jennifer Rodon Fores, Dominique Vautier, Leyla Kocgozlu, Loic Jierry, Pierre Schaaf, Fouzia Boulmedais, and Eva Harth. "Enzyme assisted peptide self-assemblies trigger cell adhesion in high density oxime based host gels." Journal of Materials Chemistry B 8, no. 20 (2020): 4419-4427.
dc.identifier.urihttps://hdl.handle.net/10657/9385
dc.language.isoeng
dc.rightsThe author of this work is the copyright owner. UH Libraries and the Texas Digital Library have their permission to store and provide access to this work. UH Libraries has secured permission to reproduce any and all previously published materials contained in the work. Further transmission, reproduction, or presentation of this work is prohibited except with permission of the author(s).
dc.subjectPolymeric Nanoparticles, Hydrogels
dc.titleDevelopment of Polymeric Networks for Drug Delivery and Tissue Engineering Applications
dc.type.dcmiText
dc.type.genreThesis
dcterms.accessRightsThe full text of this item is not available at this time because the student has placed this item under an embargo for a period of time. The Libraries are not authorized to provide a copy of this work during the embargo period.
local.embargo.lift2023-08-01
local.embargo.terms2023-08-01
thesis.degree.collegeCollege of Natural Sciences and Mathematics
thesis.degree.departmentChemistry, Department of
thesis.degree.disciplineChemistry
thesis.degree.grantorUniversity of Houston
thesis.degree.levelDoctoral
thesis.degree.nameDoctor of Philosophy

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