An application of the plating technique to the study of embryology



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As applied to embryology, the plating technique is a means for demonstrating the surface anatomy of embryos at various developmental stages. In the embryology of the chick, it is most useful for stages of development between the 15th. hour and the 9&th. hour of incubation. By this procedure, a dried embryo is placed in a vacuum chamber and plated with a metal such as chromium, silver, gold or aluminum. The effect derived is to give the embryo a skin. This makes it unnecessary to depend entirely on transparent specimens for an understanding of anatomical changes in developing embryos. The text of the paper is devoted to giving a brief outline of the techniques involved in the preparation of embryos, the plating apparatus, the plating procedures and the photography of the plated specimens. Photographs of the plated, both as monographs and stereographs, serve to illustrate the effect of metallic plating. Diagrams of typical embryos are included for comparison and diagrams of the apparatus are included for clarification. The value of plated specimens has been proven as a classroom aid. Indications are that it has wide applications in the study of comparative embryology and in volumetric ratio studies.