Synthesis of SOD1-SNAP Fusion Proteins through Molecular Cloning

The development of abnormal metal homeostasis can engender dysfunction of various neural processes and can lead to neurodegenerative diseases. Therefore, metal trafficking is highly regulated in the human body. SOD1 is an antioxidant enzyme that attaches to molecules of copper and zinc and converts reactive oxidant species into less toxic hydrogen peroxide and dioxygen. A mutant form of SOD1 could bring damages to motor neurons and cause neurodegenerative diseases such as ALS. However, the molecular mechanism of this protein is not well understood. In the present study, SOD1 was fused with a tag protein SNAP to be used in a later study of its function and molecular mechanism.