Selective probes into the mechanism of biotin action : the selection, syntheses, and spectral properties of s-methylated derivatives of thiobiotin and other biotin analogs

Simple item page
dc.contributor.advisorKohn, Harold Lewis
dc.contributor.committeeMemberCox, James R., Jr.
dc.contributor.committeeMemberDeming, Stanley N.
dc.contributor.committeeMemberMartin, Gary E.
dc.contributor.committeeMemberThummel, Randolph Paul
dc.creatorFlaster, Harry
dc.date.accessioned2023-12-07T16:12:28Z
dc.date.available2023-12-07T16:12:28Z
dc.date.issued1983
dc.description.abstractBiotin (1)) is a pivotal cofactor in numerous naturally occurring carbon dioxide transfer reactions. The detailed mechanisms of biotin dependent reactions at the molecular level are not well understood. In an effort to gain information concerning these mechanisms a series of S-methylated 2-thiobiotin based affinity labels were prepared. These substrates should yield information concerning the microenvironment of active sites in biotin dependent enzymes in general, and more specifically the biotin carboxylase (BC) and carboxyltransferase (CT) subunits of E. coll acetyl-CoA carboxylase. The rationale utilized in the selection of the desired probes is discussed. Furthermore, supportive model studies showing the feasability of synthesis as well as the likely utility of the selected probes as enzyme affinity labels are documented in this dissertation. [...]
dc.description.departmentChemistry, Department of
dc.format.digitalOriginreformatted digital
dc.format.mimetypeapplication/pdf
dc.identifier.other11125267
dc.identifier.urihttps://hdl.handle.net/10657/15554
dc.language.isoen
dc.rightsThis item is protected by copyright but is made available here under a claim of fair use (17 U.S.C. Section 107) for non-profit research and educational purposes. Users of this work assume the responsibility for determining copyright status prior to reusing, publishing, or reproducing this item for purposes other than what is allowed by fair use or other copyright exemptions. Any reuse of this item in excess of fair use or other copyright exemptions requires express permission of the copyright holder.
dc.subjectBiotin
dc.titleSelective probes into the mechanism of biotin action : the selection, syntheses, and spectral properties of s-methylated derivatives of thiobiotin and other biotin analogs
dc.type.dcmiText
dc.type.genreThesis
thesis.degree.collegeCollege of Natural Sciences and Mathematics
thesis.degree.departmentChemistry, Department of
thesis.degree.disciplineChemistry
thesis.degree.grantorUniversity of Houston
thesis.degree.levelDoctoral
thesis.degree.nameDoctor of Philosophy

Files

Original bundle

Now showing 1 - 1 of 1
Thumbnail Image
Name:
Flaster_1983_11125267.pdf
Size:
3.12 MB
Format:
Adobe Portable Document Format
Download

Collections

Digitized Theses and Dissertations (1940-2009)