The effect of carbohydrates on the transformation of Bacillus subtilis

dc.contributor.advisorEvans, John E.
dc.contributor.committeeMemberKimball, Aubrey P.
dc.contributor.committeeMemberGoldschmidt, Eugene P.
dc.contributor.committeeMemberAumann, Glenn David
dc.creatorMcLemore, Theodore L.
dc.description.abstractA study of the effect of sugars on the percent transformation in Bacillus subtilis has presented evidence which relates to the nature of competence and the competence factors. The sugars DL-glyceraldehyde, D-ribose, L-fucose, [proportional to]-lactose, and D(+)mannose were found to stimulate transformation when they were added to the transforming medium of competent Bacillus subtilis cultures. Transformation was both stimulated and inhibited when varying concentrations of D-glyceraldehyde, D(+)xylose, D(+)galactose, L-glucose,[beta]-D-glucose, L-mannose, D-fucose, D(+)glucosa- mine hydrochloride, maltose, isomaltose, and sucrose were present in the transforming medium. Inhibition of transformation was observed when varying concentrations of D-ribulose, 2-deoxy-D-ribose, o(j-D-glucose, L(-)sorbose, and D-glucose-6-phosphate were added in the presence of DNA and competent Bacillus subtilis cells. The greatest percent stimulation of transformation occurred with varying concentrations of [proportional to]-lactose, D(+)mannose, and D(+)galactose, respectively. [...]
dc.description.departmentBiology and Biochemistry, Department of
dc.format.digitalOriginreformatted digital
dc.relation.ispartofSenior Honors Theses
dc.rightsThis item is protected by copyright but is made available here under a claim of fair use (17 U.S.C. §107) for non-profit research and educational purposes. Users of this work assume the responsibility for determining copyright status prior to reusing, publishing, or reproducing this item for purposes other than what is allowed by fair use or other copyright exemptions. Any reuse of this item in excess of fair use or other copyright exemptions requires express permission of the copyright holder.
dc.titleThe effect of carbohydrates on the transformation of Bacillus subtilis
dc.type.genreThesis of Natural Sciences and Mathematics, Department of of Houston of Science


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