Kinetic behavior of glucoamylase, glucose oxidase and gluconolactonase in solution

dc.contributor.advisorBailey, James E.
dc.contributor.committeeMemberSpring, Thomas G.
dc.contributor.committeeMemberPrengle, Herman William, Jr.
dc.creatorCho, Yong Kook
dc.description.abstractThe kinetic behavior of a system of multiple enzymes in solution was studied in a variable volume batch reactor at pH 5 and T = 30 [degrees] C. The enzymes used were glucoamylase (R. delemar), glucose oxidase (A. niger), and gluconolactonase (A. niger), all of which are important commercial biocatalysts, and a dissacharide was employed as starting substrate. The study includes the basic kinetic properties of individual enzymes, interactions between components of the reaction mixture as well as the results of several detailed literature searches. The study revealed that classical Michaelis-Menten type of single substrate or two substrates kinetics predicts correctly the behavior of the sequential reaction network under conditions of constant dissolved oxygen concentration and pH. Possible industrial applications of this system was also discussed as were suggestions for further related research.
dc.description.departmentChemical and Biomolecular Engineering, Department of
dc.format.digitalOriginreformatted digital
dc.rightsThis item is protected by copyright but is made available here under a claim of fair use (17 U.S.C. Section 107) for non-profit research and educational purposes. Users of this work assume the responsibility for determining copyright status prior to reusing, publishing, or reproducing this item for purposes other than what is allowed by fair use or other copyright exemptions. Any reuse of this item in excess of fair use or other copyright exemptions requires express permission of the copyright holder.
dc.titleKinetic behavior of glucoamylase, glucose oxidase and gluconolactonase in solution
dc.type.genreThesis College of Engineering Engineering, Department of Engineering of Houston of Science


Original bundle

Now showing 1 - 1 of 1
Thumbnail Image
5.78 MB
Adobe Portable Document Format