Studies on ribonucleotide reduction in soybean callus and pea root tissue

dc.contributor.advisorCowles, Joe R.
dc.contributor.committeeMemberJurtshuk, Peter, Jr.
dc.contributor.committeeMemberSpring, Thomas G.
dc.contributor.committeeMemberVenketeswaran, S.
dc.creatorGuevara, Juan G., Jr.
dc.date.accessioned2022-06-14T20:22:23Z
dc.date.available2022-06-14T20:22:23Z
dc.date.issued1974
dc.description.abstractCell-free extracts of soybean callus and pea root tissue catalyzed the reduction of GDP to dGDP. GDP reduction by these extracts is believed to be catalyzed by ribonucleotide reductase. Crude extracts of soybean callus and pea roots catalyzed the formation of 19.0 and 0.29 nmoles of dGDP per mg protein per hour. Ribonucleotide reductase of soybean callus was purified by ammonium sulfate precipitation and Sephadex G-50 and DEAE-cellulose chromatography. Specific enzyme activity was increased approximately 7-fold. Reductase activity in a partially purified extract responded to increased amounts of protein and substrate, and increased incubation periods. Enzyme activity was not enhanced by Fe++, ATP, Mg++ or B12 coenzyme. Low concentrations of dithiothreitol and dTTP, however, stimulated reductase activity. The soybean callus and pea root extract also contained an undetermined amount of phosphatase, nucleosidase and deaminase activity.
dc.description.departmentBiology and Biochemistry, Department of
dc.format.digitalOriginreformatted digital
dc.format.mimetypeapplication/pdf
dc.identifier.other23172702
dc.identifier.urihttps://hdl.handle.net/10657/9176
dc.language.isoen
dc.rightsThis item is protected by copyright but is made available here under a claim of fair use (17 U.S.C. Section 107) for non-profit research and educational purposes. Users of this work assume the responsibility for determining copyright status prior to reusing, publishing, or reproducing this item for purposes other than what is allowed by fair use or other copyright exemptions. Any reuse of this item in excess of fair use or other copyright exemptions requires express permission of the copyright holder.
dc.titleStudies on ribonucleotide reduction in soybean callus and pea root tissue
dc.type.dcmiText
dc.type.genreThesis
thesis.degree.collegeCollege of Natural Sciences and Mathematics
thesis.degree.departmentBiology, Department of
thesis.degree.disciplineBiology
thesis.degree.grantorUniversity of Houston
thesis.degree.levelMasters
thesis.degree.nameMaster of Science

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