Variations in myocardial CPK and Na+-K+ ATPase activity following cryoprotectant exposure and low temperature storage

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1978

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Cryoprotective agents such as dimethyl sulfoxide (DMSO) and glycerol have been shown to prevent or ameliorate tissue damage during a freeze-thaw cycle. These agents have also been shown to be toxic,, to impair contraction of myocardial cells, inhibit cell growth in tissue culture and are also known to cause alterations in enzymatic activity. The purpose of this study was two-fold: a) to determine whether or not these agents afford protection during low temperature storage and b) to determine whether or not these agents affect the activity of two enzymes critical to cellular energy transduction. Creatine phosphokinase and sodium-potassium dependent adenosine triphosphatase were studied following varied intervals of normothermic and hypothermic exposure. [...]

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