Purification and electron microscopy of the microtubles of Azotobacter vinelandii strain-o

dc.contributor.advisorJurtshuk, Peter, Jr.
dc.contributor.committeeMemberFranklin, Luther E.
dc.contributor.committeeMemberLoeblich, Alfred R., III
dc.contributor.committeeMemberFox, George E.
dc.creatorMorrison, Paul R.
dc.description.abstractIt has been possible to isolate microtubules from Azotobacter vinelandii strain-o by using osmotic lysis and differential centrifugation followed by isopycnic ultracentrifugation. The microtubules found in this organism were characterized by electron microscopic analyses in penicillin G treated whole cells, thin sections of untreated cells and in an enriched, purified preparation. The microtubule, as an ultrastructure, was shown to differ from the rhapidosome by morphological analyses. Unlike rhapidosomes, microtubules do not appear to be a product of defective phage synthesis, nor can their formation be induced by mitomycin C treatment or ultraviolet irradiation. Microtubules are distinct morphological entities from the bacterial flagella. A complete morphological characterization of the A. vinelandii microtubule was made regarding dimensions, rigidity and internal structure. There was no direct attempt made to determine the function of these microtubular ultrastructures within A,, vinelandii.
dc.description.departmentBiology and Biochemistry, Department of
dc.format.digitalOriginreformatted digital
dc.rightsThis item is protected by copyright but is made available here under a claim of fair use (17 U.S.C. Section 107) for non-profit research and educational purposes. Users of this work assume the responsibility for determining copyright status prior to reusing, publishing, or reproducing this item for purposes other than what is allowed by fair use or other copyright exemptions. Any reuse of this item in excess of fair use or other copyright exemptions requires express permission of the copyright holder.
dc.titlePurification and electron microscopy of the microtubles of Azotobacter vinelandii strain-o
thesis.degree.collegeCollege of Natural Sciences and Mathematics
thesis.degree.departmentBiology, Department of
thesis.degree.grantorUniversity of Houston
thesis.degree.nameMaster of Science