Inhibition of Fatty Acid Amide Hydrolase Inhibits Tumor Growth in a Murine Model of Breast Cancer

dc.contributor.advisorMohan, Chandra
dc.contributor.committeeMemberWu, Tianfu
dc.contributor.committeeMemberBattula, Venkata Lokesh
dc.contributor.committeeMemberDu, Yong
dc.creatorSun, Jeffrey Ching-Ruey
dc.creator.orcid0000-0002-8693-1310
dc.date.accessioned2018-03-12T20:15:37Z
dc.date.available2018-03-12T20:15:37Z
dc.date.createdDecember 2017
dc.date.issued2017-12
dc.date.submittedDecember 2017
dc.date.updated2018-03-12T20:15:38Z
dc.description.abstractIt has been shown that endocannabinoids exert a pro-apoptotic effect on cancer cell lines which typically have mutated apoptosis mechanisms. It is known that FAAH is a downstream regulator of endocannabinoid levels which degrades them into inactive fatty acids and ethanolamine. By inhibiting FAAH using the inhibitor URB 597, we can harness the body’s natural production of endocannabinoids to encourage apoptosis in cancer cells. We have shown through in vitro models that FAAH is present in breast cancer and increased apoptosis can be achieved through the addition of exogenous endocannabinoids and URB 597. Here, we develop an in vitro model of breast cancer using GFP & luciferase transduced breast cancer cells and an immune-deficient mouse model. After establishing breast cancer tumors and treating with URB 597, we are able to show that the inhibition of FAAH also results in decreased proliferation and increased apoptosis in breast cancer cells in vivo.
dc.description.departmentBiomedical Engineering, Department of
dc.format.digitalOriginborn digital
dc.format.mimetypeapplication/pdf
dc.identifier.urihttp://hdl.handle.net/10657/2927
dc.language.isoeng
dc.rightsThe author of this work is the copyright owner. UH Libraries and the Texas Digital Library have their permission to store and provide access to this work. Further transmission, reproduction, or presentation of this work is prohibited except with permission of the author(s).
dc.subjectBreast cancer
dc.subjectMouse Model
dc.subjectApoptosis
dc.subjectEndocannabinoids
dc.subjectProliferation
dc.subjectFatty Acid Amide Hydrolase
dc.subjectFAAH
dc.subjectEGFR
dc.subjectURB 597
dc.subjectMatrigel
dc.subjectIn vivo
dc.subjectIn vitro
dc.titleInhibition of Fatty Acid Amide Hydrolase Inhibits Tumor Growth in a Murine Model of Breast Cancer
dc.type.dcmiText
dc.type.genreThesis
local.embargo.lift2018-12-01
local.embargo.terms2018-12-01
thesis.degree.collegeCullen College of Engineering
thesis.degree.departmentBiomedical Engineering, Department of
thesis.degree.disciplineBiomedical Engineering
thesis.degree.grantorUniversity of Houston
thesis.degree.levelMasters
thesis.degree.nameMaster of Science

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