Effects of proteinase inhibitors on the differentiation process of physarum flavicomum : isolation of proteinases from growing and encysting cells

dc.contributor.committeeMemberHenney, Henry R., Jr.
dc.contributor.committeeMemberFranklin, Luther E.
dc.contributor.committeeMemberSizemore, Ronald K.
dc.contributor.committeeMemberGeanangel, Russell A.
dc.creatorTavana, Ghamartaj
dc.date.accessioned2024-01-02T20:06:03Z
dc.date.available2024-01-02T20:06:03Z
dc.date.issued1980
dc.description.abstractThe use of several inhibitors of proteinases has revealed the inhibition by some and delay by others of the differentiation process of Physarum flavicomum haploid cells. Among these inhibitors TLCK was the first (inhibiting encystment in both SD medium and BS solution) and antipain (inhibiting encystment in SD medium) the second most effective inhibitor of the encystment process. Chloroquine was also a potent inhibitor preventing differentiation in SD medium. Leupeptin, pepstatin and the growth factor hemin all delayed the encystment process in SD medium. The proteinases were isolated from growing cells, and cells that had started to differentiate (5% cysts) in SD medium, by affinity chromatography. All of the inhibitors were tested for the inhibitory effects upon the crude extract from both growing and differentiating cells of P. flavicomum in SD medium using Azocoll and Azocasein as subtrate. All inhibitors were found to be effective in blocking part of the proteolytic activity of the crude extract with different ranges. For each inhibitor the inhibition was found to be concentration dependent.
dc.description.departmentBiology and Biochemistry, Department of
dc.format.digitalOriginreformatted digital
dc.format.mimetypeapplication/pdf
dc.identifier.other7783119
dc.identifier.urihttps://hdl.handle.net/10657/15702
dc.language.isoen
dc.rightsThis item is protected by copyright but is made available here under a claim of fair use (17 U.S.C. Section 107) for non-profit research and educational purposes. Users of this work assume the responsibility for determining copyright status prior to reusing, publishing, or reproducing this item for purposes other than what is allowed by fair use or other copyright exemptions. Any reuse of this item in excess of fair use or other copyright exemptions requires express permission of the copyright holder.
dc.subjectEnzyme inhibitors
dc.subjectCells
dc.titleEffects of proteinase inhibitors on the differentiation process of physarum flavicomum : isolation of proteinases from growing and encysting cells
dc.type.dcmiText
dc.type.genreThesis
thesis.degree.collegeCollege of Natural Sciences and Mathematics
thesis.degree.departmentBiology, Department of
thesis.degree.disciplineBiology
thesis.degree.grantorUniversity of Houston
thesis.degree.levelMasters
thesis.degree.nameMaster of Science

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