Growth of amoebae in a partially-defined medium

dc.contributor.advisorHenney, Henry R., Jr.
dc.contributor.committeeMemberKimball, Aubrey P.
dc.contributor.committeeMemberGoldschmidt, Eugene P.
dc.contributor.committeeMemberAumann, Glenn David
dc.creatorCatrett, Edith
dc.description.abstractAmoebae, tentatively identified, as myxamoebae of Physarum flavicomum variety 1, were grown in the absence of bacteria on a partially-defined medium. The medium consisted of basal salts, 1.5% proteose peptone, 0.5% yeast extract, 0.5% glucose, and 2.5 ug per ml hematin. Typically a yield of 1 x 10^6 cells/ml was obtained, with a generation time of approximately 89 hr. The optimum temperature for growth was 23 C. Aeration by shaking at 170 rev/min stimulated growth dramatically, as compared to stationary and anaerobic conditions. Hematin has not yet been established as an absolute requirement of the amoebae. The optimum pH for growth is 4.6. Amoebae grown on the partially-defined medium did not form plasmodia.
dc.description.departmentBiology and Biochemistry, Department of
dc.format.digitalOriginreformatted digital
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dc.titleGrowth of amoebae in a partially-defined medium
dc.type.genreThesis of Arts and Sciences, Department of of Houston of Science


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