Growth of amoebae in a partially-defined medium

dc.contributor.advisorHenney, Henry R., Jr.
dc.contributor.committeeMemberKimball, Aubrey P.
dc.contributor.committeeMemberGoldschmidt, Eugene P.
dc.contributor.committeeMemberAumann, Glenn David
dc.creatorCatrett, Edith
dc.date.accessioned2022-02-21T16:02:21Z
dc.date.available2022-02-21T16:02:21Z
dc.date.issued1972
dc.description.abstractAmoebae, tentatively identified, as myxamoebae of Physarum flavicomum variety 1, were grown in the absence of bacteria on a partially-defined medium. The medium consisted of basal salts, 1.5% proteose peptone, 0.5% yeast extract, 0.5% glucose, and 2.5 ug per ml hematin. Typically a yield of 1 x 10^6 cells/ml was obtained, with a generation time of approximately 89 hr. The optimum temperature for growth was 23 C. Aeration by shaking at 170 rev/min stimulated growth dramatically, as compared to stationary and anaerobic conditions. Hematin has not yet been established as an absolute requirement of the amoebae. The optimum pH for growth is 4.6. Amoebae grown on the partially-defined medium did not form plasmodia.
dc.description.departmentBiology and Biochemistry, Department of
dc.format.digitalOriginreformatted digital
dc.format.mimetypeapplication/pdf
dc.identifier.other17885677
dc.identifier.urihttps://hdl.handle.net/10657/8879
dc.language.isoen
dc.rightsThis item is protected by copyright but is made available here under a claim of fair use (17 U.S.C. §107) for non-profit research and educational purposes. Users of this work assume the responsibility for determining copyright status prior to reusing, publishing, or reproducing this item for purposes other than what is allowed by fair use or other copyright exemptions. Any reuse of this item in excess of fair use or other copyright exemptions requires express permission of the copyright holder.
dc.titleGrowth of amoebae in a partially-defined medium
dc.type.dcmiText
dc.type.genreThesis
thesis.degree.collegeCollege of Arts and Sciences
thesis.degree.departmentBiology, Department of
thesis.degree.disciplineBiology
thesis.degree.grantorUniversity of Houston
thesis.degree.levelMasters
thesis.degree.nameMaster of Science

Files

Original bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
Catrett_1972_17885677.pdf
Size:
2.25 MB
Format:
Adobe Portable Document Format