Pigment mutation allows competition experiments to assess fitness in Micrococcus luteus

dc.contributorWidger, William R.
dc.contributor.authorZimmerer, Rene
dc.date.accessioned2019-01-02T21:33:31Z
dc.date.available2019-01-02T21:33:31Z
dc.date.issued2018-10-18
dc.description.abstractDormancy is a growth state some bacteria use to survive antibiotic treatment and cause latent infections. Existing antibiotic treatment could be more effective if one could prevent bacteria from entering a dormant state, however potential target genes that regulate dormancy are unknown. From the yellow wild-type Micrococcus luteus (ML), we have developed a colorless mutant (delta-crtE::kan). This mutant can be used to perform competition experiments probing phenotypes of genes regulating dormancy. Competition experiments using ML are significant because they allow for a method comparing the growth of wild-type ML to mutants with knockouts in suspected regulatory genes of dormancy. My project is to demonstrate the pigment mutation in delta-crtE::kan is at a neutral site and does not affect its growth. Results of these experiments show the delta-crtE::kan mutant has similar growth and fitness to wild-type allowing the delta-crtE::kan mutant to act as a substitute for wild-type in future competition experiments. One such experiment is between delta-crtE and a universal stress protein (delta-uspA616::kan) implicated in dormancy. Preliminary data shows the delta-uspA616::kan mutant ML is outcompeted by delta-crtE::kan in nutrient poor media.
dc.description.abstractDormancy is a growth state some bacteria use to survive antibiotic treatment and cause latent infections. Existing antibiotic treatment could be more effective if one could prevent bacteria from entering a dormant state, however potential target genes that regulate dormancy are unknown. From the yellow wild-type Micrococcus luteus (ML), we have developed a colorless mutant (delta-crtE
dc.identifier.urihttp://hdl.handle.net/10657/3783
dc.language.isoen_US
dc.relation.ispartofSummer Undergraduate Research Fellowship
dc.rightsThe author of this work is the copyright owner. UH Libraries and the Texas Digital Library have their permission to store and provide access to this work. Further transmission, reproduction, or presentation of this work is prohibited except with permission of the author(s).
dc.titlePigment mutation allows competition experiments to assess fitness in Micrococcus luteus
dc.typePoster

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