A fine structural study of sperm differentiation and the effects of colchicine on sperm morphology in Nematospiroides dubius



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An ultrastructural study of N. dubius sperm differentiation and the effects of colchicine on this process are presented. The spermatogonia and primary spermatocytes are attached to a cytoplasmic nucleated rachis. Early spermatogonia have a large nucleus with one or two nucleoli. The cytoplasm contains ribosomes, microtubules, lipid-like droplets, and endoplasmic reticulum. In more advanced spermatogonia, Golgi bodies become abundant and continuity between the outer nuclear membrane and endoplasmic reticulum is common. Cisternae of the endoplasmic reticulum are often noted surrounding masses of fibrillar material. In such cases one side of the reticular cisternae becomes hypertrophied. Primary spermatocytes exhibit an increased amount of endoplasmic reticulum and fibrillar material. Both components collectively make the fibrillar bodies which, at this stage, are scattered throughout the cytoplasm. Secondary spermatocytes are joined to each other by cytoplasmic bridges. In these cells the fibrillar bodies and other organelles are localized in the peripheral cytoplasm. A limited number of microtubules are present in perinuclear cytoplasm. [...]