Mode of action of harveyicin, the bacteriocin of a marine luminescent bacterium Vibrio harveyi

Date
1981
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Abstract

The first marine bacteriocinogenic bacterium, Vibrio (Beneckea) harveyi, has recently been discovered and the bacteriocin, called harveyicin, has been partially characterized. However, the mode of action of this bacteriocin was unknown. The objectives of this study were to maximize harveyicin production, to purify the bacteriocin, and to study the mode of action of the lethal effect of harveyicin. Harveyicin production was found to be time dependent especially on solid media such as SWYE agar medium. The time during which harveyicin remained active was greatly affected by the age of the medium. Harveyicin was shown to be sensitive to a protease produced by the harveyicinogenic strain and the loss of the bacteriocin with extended incubation was postulated to be due to proteolytic degradation. The salinity of growth media also affected harveyicin titers and a salinity lower than full strength seawater gave maximal harveyicin production. Attempts to induce harveyicin production with mitomycin C, ultraviolet light, and anaerobic medium did not give satisfactory results. Harveyicin was found to be a stable substance which could be concentrated by common protein purification schemes. [...]

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Keywords
Bacteriocins, Harveyicin
Citation