Biophysical studies of key-hole limpet hemocyanin in salt solutions

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1967

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Abstract

The dissociation of Key-Hole limpet hemocyanin (KLH) has been studied as a function of potassium bromide concentration. In general, KLH was found to dissociate in concentrated KBr in a similar manner to the way it dissociates in basic solutions. However, there are several differences in the physical properties of the different subunits. It appears that the dissociative effect of KBr and base are additive. Therefore, when KLH is dissociated with potassium bromide it is at a lower pH than when it is dissociated with base and less highly charged. The less highly charged subunits have higher sedimentation coefficients presumably because they are more compact.

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