The Diagnostic Potential of Stool Proteins Outperforms Plasma Proteins in Inflammatory Bowel Disease



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Background: Inflammatory bowel disease (IBD) is a group of autoimmune disorders defined by chronic inflammation of the gastrointestinal tract. Crohnï¾’s disease (CD) and Ulcerative Colitis (UC) are the two types of IBD. Biomarkers can be used as a diagnostic tool and for regular monitoring to analyze disease progression in IBD patients. Methods: The samples were obtained from Emory University School of Medicine, Division of Pediatric Gastroenterology . Data analysis was conducted on the biomarkers of the samples using GraphPad Prism7, Microsoft Excel, easyROC software. There were 1129 proteins from 11 paired samples 6 CD, 5 UC, 1 HC) in the cohort. Results: In a comparison between the 11 paired stool and plasma biomarker samples from our data analysis, 305 stool proteins have a p-value of < 0.05 in a u test while plasma samples have only 90 proteins. Furthermore, there are 134 stool proteins that have a fold change of > 2 while there are only 14 plasma samples with FC > 2. Lastly, 47 proteins have a ROC AUC value of above 0.9 while there are no plasma biomarkers with ROC AUC values > 0.9. Conclusion: Stool biomarkers are better indicators of IBD compared to plasma biomarkers. Keywords: IBD, UC, CD, stool biomarkers, FC, ROC AUC