Multifunctional CuS Nanoparticles for Imaging Guided Photothermal Ablation Therapy

dc.contributor.advisorChow, Diana Shu-Lian
dc.contributor.committeeMemberHu, Ming
dc.contributor.committeeMemberWilliams, Louis
dc.contributor.committeeMemberLi, Chun
dc.contributor.committeeMemberStafford, R. Jason
dc.creatorCui, Lili
dc.date.accessioned2018-03-02T21:11:14Z
dc.date.available2018-03-02T21:11:14Z
dc.date.createdMay 2015
dc.date.issued2015-05
dc.date.submittedMay 2015
dc.date.updated2018-03-02T21:11:14Z
dc.description.abstractStatement of the problem: A process for rapid synthesis of radioactive [64Cu]CuS nanoparticles was successfully developed in our collaborator, Dr. Chun Li’s group. Further investigation shows that [64Cu]CuS nanoparticles can act as an efficient radiotracer for PET imaging, and can be used for photothermal ablation therapy using near-infrared laser irradiation (Zhou, Zhang et al. 2010). Based on these novel [64Cu]CuS NPs, we have designed and investigated two different types of NPs for targeting delivery and dual imaging agents. Methods: The NPs were charaterized with particle size, zeta potential, UV-vis spectroscopy, and photothermal effect in aqueous solution. The cytotoxicity was tested in HepG2, HEK293 and U87 cell lines. The tumor binding activity and the photothermal effect for the targeted CuS NPs was evaluated in U87 cells. PET/CT imaging, biodistribution and PTA therapy were performed in a U87 xenograft mouse tumor model. For the doped NPs, the elements quantification, MRI relaxivity and MRI imaging were also investigated. Results: The tumor uptakes at 24 h after i.v. injection for targeted [64Cu]CuS NPs and [64Cu]CuS-PEG NPs was about 10% and 5% ID/g, respectively. The PTA results revealed the 100 % necrotic area in tumor for the mice treated with targeted CuS NPs, compared with the 60% necrotic area for the ones treated with non-targeted CuS NPS. The MRI relaxivity was calculated based on the concentration of Mn ions. The r1 and r2 values were 5.9 (s mM)-1 and 110.7 (s mM)-1, respectively. The PET/CT imaging confirmed the accumulation of the radiolabeled [64Cu][Mn]CuS-PEG NPs in the tumor 24 h after i.v. injection. [Mn]CuS-PEG NPs exhibited 80% of necrotic area in tumor after PTA therapy.
dc.description.departmentPharmacological and Pharmaceutical Sciences, Department of
dc.format.digitalOriginborn digital
dc.format.mimetypeapplication/pdf
dc.identifier.urihttp://hdl.handle.net/10657/2753
dc.language.isoeng
dc.rightsThe author of this work is the copyright owner. UH Libraries and the Texas Digital Library have their permission to store and provide access to this work. Further transmission, reproduction, or presentation of this work is prohibited except with permission of the author(s).
dc.subjectNanoparticles
dc.subjectPhotothermal ablation therapy
dc.subjectCuS
dc.titleMultifunctional CuS Nanoparticles for Imaging Guided Photothermal Ablation Therapy
dc.type.dcmiText
dc.type.genreThesis
thesis.degree.collegeCollege of Pharmacy
thesis.degree.departmentPharmacological and Pharmaceutical Sciences, Department of
thesis.degree.disciplinePharmaceutics
thesis.degree.grantorUniversity of Houston
thesis.degree.levelDoctoral
thesis.degree.nameDoctor of Philosophy

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