The induction of Pl phage in E. coli thy-(Pl)

dc.contributor.advisorJones, Roosevelt J.
dc.contributor.committeeMemberCowles, Joe R.
dc.contributor.committeeMemberGray, Horace B., Jr.
dc.contributor.committeeMemberAumann, Glenn David
dc.creatorKeating, Robert Joseph
dc.date.accessioned2022-02-21T16:02:28Z
dc.date.available2022-02-21T16:02:28Z
dc.date.issued1972
dc.description.abstractThis investigation has shown that analogs of thymine induced Pl phage from E. coli C thy-3284. The Pl phage was induced by the antibiotic mitomycin C and thymine starvation. The induction of Pl phage from strain C-328 by uracil derivatives indicates that direct interference with DNA synthesis is not necessary for Pl prophage induction. UV irradiation, which caused phage induction and lysis in the lambda system, did not induce the Pl phage from C-328. This suggests a difference between the two phage systems. Inhibition of protein synthesis by chloroamphenicol did not induce Pl phage from C-328. An alteration in certain physical conditions, pH and osmotic pressure failed to induce Pl phage. When E. coli C thy-3284 (Pl) was incubated at 45°C, a bi- modal growth response was observed. The significance of this bimodal response was associated with the presence of two phage types: a thermoinducible and non-thermoinducible Pl phage in the culture of C-328. It was shown that E. coli C thy-3284 (Pl) was lysogenized by two phage types, such that some cells were lysogenized by the thermoinducible phage while others were lysogenized by the non-thermoinducible phage. It is suggested that one phage type was probably a mutant of the parent phage.
dc.description.departmentBiology and Biochemistry, Department of
dc.format.digitalOriginreformatted digital
dc.format.mimetypeapplication/pdf
dc.identifier.other17972124
dc.identifier.urihttps://hdl.handle.net/10657/8883
dc.language.isoen
dc.rightsThis item is protected by copyright but is made available here under a claim of fair use (17 U.S.C. §107) for non-profit research and educational purposes. Users of this work assume the responsibility for determining copyright status prior to reusing, publishing, or reproducing this item for purposes other than what is allowed by fair use or other copyright exemptions. Any reuse of this item in excess of fair use or other copyright exemptions requires express permission of the copyright holder.
dc.titleThe induction of Pl phage in E. coli thy-(Pl)
dc.type.dcmiText
dc.type.genreThesis
thesis.degree.collegeCollege of Arts and Sciences
thesis.degree.departmentBiology, Department of
thesis.degree.disciplineBiology
thesis.degree.grantorUniversity of Houston
thesis.degree.levelMasters
thesis.degree.nameMaster of Science

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