A study of conjugation in E. coli as a function of the F-cell division cycle

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1970

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Abstract

The recombinant formation in the conjugation system was studied as a function of the cell division cycle of synchronous F- cultures. In this study, one F- (3704) strain Ileu-, Leu-, pro- and lys- and three Hfr strains were used. The synchronous F- (3704) and Hfr G10 recombinants showed a periodic increase in a sequential order for isoleucine, leucine and proline markers with the synchronous F- cell division cycle. The synchronous F-(3704) and Hfr 3851 recombinants also indicated a periodic increase in a sequential order for lysine, proline, leucine and isoleucine markers with the synchronous F- cell division cycle. This indicates that if a large portion of DNA is transferred either in clockwise or counterclockwise direction into the synchronous F- cells, the recombinants for the markers are formed in the same sequential order as they entered into F- cells. [...]

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