Interaction between Liver X Receptor Beta and Estrogen Receptor Alpha Variants in vitro

Abstract

This project tests the interaction between the protein Liver X Receptor beta (LXR-beta) and variants of Estrogen Receptor alpha (ER-alpha) proteins in the presence of different ligands. The plasmids were modified in the lab and proteins were made from these plasmids using the coupled transcription/translation system. The techniques used were Co-Immunoprecipitation and Western Blot. The result shows interesting and unexpected interactions between LXR-beta and ER-alpha variants. More study is needed to measure the specific affinity of LXR-beta to each ER-alpha variant and the effect of these complexes.

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