• Login
    View Item 
    •   Repository Home
    • Electronic Theses and Dissertations
    • Published ETD Collection
    • View Item
    •   Repository Home
    • Electronic Theses and Dissertations
    • Published ETD Collection
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Assessing Teratogen-Induced Changes in Murine Fetal Brain Vasculature Using In Utero Optical Coherence Tomography

    View/Open
    RAGHUNATHAN-DISSERTATION-2020.pdf (3.233Mb)
    Date
    2020-05
    Author
    Raghunathan, Raksha
    0000-0002-2434-425X
    Metadata
    Show full item record
    Abstract
    This dissertation reports the use of in utero optical coherence tomography to evaluate changes in vasculature in a developing murine fetal brain caused due to prenatal exposure to teratogens. Embryogenesis is a highly complex process that is extremely vulnerable to external factors. Proper visualization of embryonic development is crucial to understand the basic physiological processes and identify defects if any. This dissertation is divided into two major sections: 1) assessing teratogen induced changes in the murine fetal brain vasculature during the second trimester equivalent period (chapters 2-4) and 2) combining optical coherence tomography with Brillouin microscopy to image and evaluate changes in biomechanical properties during neural tube closure in order to study first trimester exposure to teratogens (appendix A3). The first section is further divided into the following sub-sections: 1) assessing alcohol induced changes in murine fetal brain vasculature, 2) assessing nicotine induced changes in murine fetal brain vasculature, and 3) assessing synthetic cannabinoid (SCB) induced changes in murine fetal brain vasculature. The advancement of algorithms to image and detect minute changes in vasculature are also detailed. The contributions of this work are significant to understand the effects of teratogens on development, as blood flow plays a major role in embryogenesis. Understanding the acute changes in vasculature caused within minutes of maternal exposure to a teratogen can open several new avenues to explore as blood flow drives organ development. Results from this dissertation have been published in 7 first author peer-reviewed publications.
    URI
    https://hdl.handle.net/10657/6691
    Collections
    • Published ETD Collection

    DSpace software copyright © 2002-2016  DuraSpace
    Contact Us | Send Feedback
    TDL
    Theme by 
    Atmire NV
     

     

    Browse

    All of DSpaceCommunities & CollectionsBy Issue DateAuthorsDepartmentsTitlesSubjectsThis CollectionBy Issue DateAuthorsDepartmentsTitlesSubjects

    My Account

    Login

    DSpace software copyright © 2002-2016  DuraSpace
    Contact Us | Send Feedback
    TDL
    Theme by 
    Atmire NV