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    Permeability of anti-fouling PEGylated surfaces probed by fluorescence correlation spectroscopy

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    Willson_2017_PermeabilityofAnti-FoulingAM.pdf (869.3Kb)
    Date
    2017-10
    Author
    Daniels, Charlisa R.
    Reznik, Carmen
    Kilmer, Rachel
    Felipe, Mary J.
    Tria, Maria C. R.
    Kourentzi, Katerina D.
    Chen, Wen-Hsiang
    Advincula, Rigoberto C.
    Willson, Richard C.
    Landes, Christy F.
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    Abstract
    The present work reports on in situ observations of the interaction of organic dye probe molecules and dye-labeled protein with different poly(ethylene glycol) (PEG) architectures (linear, dendron, and bottle brush). Fluorescence correlation spectroscopy (FCS) and single molecule event analysis were used to examine the nature and extent of probe朠EG interactions. The data support a sieve-like model in which size-exclusion principles determine the extent of probe朠EG interactions. Small probes are trapped by more dense PEG architectures and large probes interact more with less dense PEG surfaces. These results, and the tunable pore structure of the PEG dendrons employed in this work, suggest the viability of electrochemically-active materials for tunable surfaces.
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    https://hdl.handle.net/10657/6222
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