An Engineered Protease that Cleaves Specifically after Sulfated Tyrosine

Date

4/1/2009

Journal Title

Journal ISSN

Volume Title

Publisher

Angewandte Chemie International Edition

Abstract

The bacterial protease OmpT has been engineered to cleave after sulfotyrosine residues in peptide substrates but not after phosphotyrosine (see scheme). A selection/counterselection screen was used to identify OmpT variants with the desired specificity and high levels of overall catalytic activity.

Description

Keywords

post-translational modifcation, proteases, protein engineering

Citation

Copyright 2009 Angewandte Chemie International Edition . This is a post-print version of a published paper that is available at: http://ev7su4gn4p.search.serialssolutions.com/?id=doi:10.1002/anie.200800736&sid=wiley&iuid=7265662&date=2008&jtitle=Angewandte+Chemie+International+Edition&volume=47&atitle=An+Engineered+Protease+that+Cleaves+Specifically+after+Sulfated+Tyrosine&genre=article&spage=7861&issue=41&title=Angewandte+Chemie+International+Edition&issn=1433-7851&epage=7863. Recommended citation: Varadarajan, Navin, George Georgiou, and Brent L. Iverson. "An engineered protease that cleaves specifically after sulfated tyrosine." Angewandte Chemie International Edition 47, no. 41 (2008): 7861-7863. doi: 10.1002/anie.200800736.This item has been deposited in accordance with the publisher copyright and licensing terms with the author's permission.