An Engineered Protease that Cleaves Specifically after Sulfated Tyrosine
Iverson, Brent L.
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The bacterial protease OmpT has been engineered to cleave after sulfotyrosine residues in peptide substrates but not after phosphotyrosine (see scheme). A selection/counterselection screen was used to identify OmpT variants with the desired specificity and high levels of overall catalytic activity.