Discovery and Validation of IgG4 Anti-Gliadin Autoantibody as a Disease Marker of Psoriasis

Antigen microarray is a high-throughput technology, which can be used to select potential biomarkers. In this paper, antigen microarray is the main technology used to determine potential autoantibody biomarkers in different diseases: psoriasis and breast cancer. IgG subtype and IgG total were used as secondary antibodies to bind autoantibodies in patients’ serum, for psoriasis and breast cancer respectively. The enzyme-linked immunosorbent assay (ELISA) was adopted to measure serum level of the potential autoantibodies in a large number of serum samples from patients and healthy controls. These studies have revealed IgG4 anti-human gliadin autoantibody as a potential novel biomarker of psoriasis.