Bacterial Consortium and Effect of Different Conditions on Cell Growth Using Naphthalene as a Sole Carbon Source by the Bacteria Isolated from Soil Samples Collected from Oil Refineries in Texas

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2017-05

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Abstract

Petrochemical refineries and human activities are responsible for release of carcinogenic compounds known as Polycyclic Aromatic Hydrocarbons (PAHs). Adverse effects of these compounds on human health and ecosystem resulted in an increased awareness, and lead to dramatic increase in research intended to remove PAHs from the ecosystem. In the present study naphthalene was used as a model compound. It was hypothesized that the microorganisms isolated from naphthalene contaminated soil collected from petrochemical refineries in Texas could be utilizing naphthalene as a carbon source. The naphthalene degradation capability of microorganisms could be an indication of their ability to degrade PAHs. In this study the isolated strains were identified as Enterobacter cloacae and Cellulosimicrobium cellulans based on results from 16S rDNA gene sequencing analysis. Bacterial consortium and effect of different conditions on cell growth using naphthalene as a sole carbon source were studied.

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Keywords

Polycyclic Aromatic Hydrocarbons, Bacterial consortium, Naphthalene, Enterobacter cloacae, Cellulosimicrobium cellulans, Soil, Oil refineries

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